Ribonucleotide reductase metallocofactor: assembly, maintenance and inhibition
نویسندگان
چکیده
منابع مشابه
Conserved electron donor complex Dre2-Tah18 is required for ribonucleotide reductase metallocofactor assembly and DNA synthesis.
Eukaryotic ribonucleotide reductases (RNRs) require a diferric-tyrosyl radical (Fe(III)2-Y•) cofactor to produce deoxynucleotides essential for DNA replication and repair. This metallocofactor is an important target of RNR-based therapeutics, although mechanisms of in vivo cofactor assembly, inactivation, and reactivation are poorly understood. Here, we demonstrate that the conserved Fe-S prote...
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suppresses DNAsynthesis by inhibition of ribonucleotide reductase.4) Ribonucleotide reductase plays an essential role in DNAsynthesis in most organisms.5) The enzyme is a nonheme iron-containing protein, which requires ATPand Mg2+ as co factors. As previously reported that IC202C has art affinity against Fe(III) ion,3) we have examined the effect of IC202C on ribonucleotide reductase activity u...
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In many organisms, hydroxyurea (HU) inhibits class I ribonucleotide reductase, leading to lowered cellular pools of deoxyribonucleoside triphosphates. The reduced levels for DNA precursors is believed to cause replication fork stalling. Upon treatment of the hyperthermophilic archaeon Sulfolobus solfataricus with HU, we observe dose-dependent cell cycle arrest, accumulation of DNA double-strand...
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oncogenic RNA viruses. It has also been reported (24) that single-stranded polyribonucleotides at very low concentrations inhibit DNA polymerase of Rauscher leukemia virus. In this report, we present data that show a way in which RNA indirectly affects DNA synthesis. These data show that RNA markedly inhibits ribonucleotide reducÃ-asefrom Ehrlich ascites tumor cells, at concentrations which cou...
متن کاملInhibition of ribonucleotide reductase from Ehrlich tumor cells by RNA.
oncogenic RNA viruses. It has also been reported (24) that single-stranded polyribonucleotides at very low concentrations inhibit DNA polymerase of Rauscher leukemia virus. In this report, we present data that show a way in which RNA indirectly affects DNA synthesis. These data show that RNA markedly inhibits ribonucleotide reducÃ-asefrom Ehrlich ascites tumor cells, at concentrations which cou...
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ژورنال
عنوان ژورنال: Frontiers in Biology
سال: 2014
ISSN: 1674-7984,1674-7992
DOI: 10.1007/s11515-014-1302-6